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The newest attL fragment is actually PCR amplified out of pCMVssattL having fun with primers attL(BOP?)Fwd-(KpnI) and attL(BOP?)Rev-(NotI)

Posted by Prabhjot7 jot7
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On May 4, 2022

The newest attL fragment is actually PCR amplified out of pCMVssattL having fun with primers attL(BOP?)Fwd-(KpnI) and attL(BOP?)Rev-(NotI) pTZ-attP4X-UN-EF1?-eGFP try from subcloning brand new attP4X sequence away from pattP4X-PGKssPuro because the a keen EcoRI fragment on novel EcoRI webpages upstream of one's person UTF1 promoter when you look at the

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